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ADVANCED OXIDATION TECHNOLOGIES

2000 - 2009 TEST RESULTS

H1N1 (Swine Flu)

Kansas State University has completed preliminary testing on RGF’s Photohydroionization

(PHI-Cell®) and Reflective Electromagnetic Energy (REME® Cell) technologies with

99+% inactivation of H1N1 Swine Flu on a stainless steel surface. Further tests are

scheduled. 2009 H1N1 (referred to as “swine flu” early on) is a new influenza virus causing

illness in people. This new virus was first detected in people in the United States in

April 2009. This virus is spreading from person-to-person worldwide. On June 11, 2009,

the World Health Organization (WHO) signaled that a pandemic of 2009 H1N1 flu was

underway. Spread of 2009 H1N1 virus is thought to occur in the same way that seasonal

flu spreads. Flu viruses are spread mainly from person to

person through coughing or sneezing by people with influenza.

Sometimes people may become infected by touching

items – such as a surface or object – with flu viruses on

it and then touching their mouth or nose.

Norwalk Virus

Noroviruses are a group of related, single-stranded RNA, nonenveloped viruses that

cause acute gastroenteritis in humans. Noroviruses are named after the original strain

"Norwalk virus," which caused an outbreak of gastroenteritis in a school in Norwalk,

Ohio, in 1968. No evidence suggests that infection occurs through the respiratory system.

Noroviruses are highly contagious and as few as 10 viral particles may be sufficient

to infect an individual. During outbreaks of norovirus, several modes of transmission

have been documented; for example, initial food-borne transmission in a restaurant,

followed by secondary person-to-person transmission to household contacts. 50% of

all food-borne outbreaks of gastroenteritis can be attributed

to noroviruses. Among the 232 outbreaks of norovirus illness

reported to CDC from 1997 to 2000 36% were in

restaurants, 23% were in nursing homes, 13% were in

schools and 10% were vacation settings or cruise ships.

Source: CDC-Centers for Disease Control and Prevention

Tested by Midwest Research Institute Inactivation Rate 99+% 1

Avian influenza (Bird Flu)

Avian influenza is an infection caused by avian (bird) influenza (flu) viruses. These influenza

viruses occur naturally among birds. Wild birds worldwide carry the viruses in

their intestines, but usually do not die from them. However, avian influenza is very contagious

among birds and can make some domestic birds, including chickens, ducks and

turkeys, very sick and kill them. Of the few avian influenza viruses that have crossed

the species barrier to infect humans, H5N1 has had the largest number of detected

cases of severe disease and death in humans. Of the

human cases associated with the ongoing H5N1 outbreaks

in poultry and wild birds in Asia, Europe, the Near East and

Africa, more than half of those people reported infected with

the virus died. While there has been some human-tohuman

spread in H5N1, it has been limited, inefficient and

unsustained.

Source:CDC: Center for Disease Control and Prevention

Tested by Kansas State UniversityInactivation Rate 99+%

Tested by Kansas State University Inactivation Rate 99+%

RGF first developed its Advanced Oxidation Technology over 20 years ago. Over 1 million RGF Cells are in use around the

world. RGF has licensed its technology to many Fortune 500 companies for use in the medical, food, military, residential,

commercial, marine, hospitality and government, etc. RGF cells in various products have been tested and/or approved or

registered by:

In addition, RGF cells have been specified in the Norovirus & MRSA protection plan of America’s largest restaurant chains,

hotel chains, theme parks, cruise lines, public schools and hospitals. The following is a summary of some of the testing and

studies performed by third party independent labs and universities. RGF products are not medical devices and no medical

claims are made.

•U.S. Government – GSA

•European Union

•USDA & FSIS

•UL, ETL, TUV, EU, EPA & CSA

•U.S. Military

•Electric Power Research Institute

•Chinese Government

•Japanese Government (TV commercials)

•Canadian Government

Streptococcus Sp.

Group A Streptococcal (strep) infections are caused by group A streptococcus, a

bacterium responsible for a variety of health problems. These infections can range

from mild skin infection or sore throat to severe, life-threatening conditions such as

toxic shock syndrome and necrotizing fasciitis, commonly known as flesh eating disease.

Health experts estimate that more than 10 million mild infections (throat and

skin) like these occur every year. Secondary infections

include: Rheumatic Fever, Impetigo, Cellulties,

Erysipelas and Scarlet Fever.

Source: U.S. DEPARTMENT OF HEALTH AND HUMAN

SERVICES

Tested by Kansas State University Inactivation Rate 96+%

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Listeria

This is a Gram-positive bacterium, motile by means of flagella. Some studies suggest

that 1-10% of humans may be intestinal carriers of L. monocytogenes. It has

been found in at least 37 mammalian species, both domestic and feral, as well as

at least 17 species of birds and possibly some species of fish and shellfish. The

manifestations of listeriosis include septicemia, meningitis (or meningoencephalitis),

encephalitis, and intrauterine or cervical infections in

pregnant women, which may result in spontaneous abortion

or stillbirth.

Source: U.S. Food and Drug Administration

Tested by Kansas State University

Steris Labs

KAG / Eco Labs

Inactivation Rate 99+%

Escherichia coli

Escherichia coli, usually abbreviated to E. coli, discovered by Theodor Escherich,

a German pediatrician and bacteriologist, is one of the main species of bacteria

that live in the lower intestines of mammals, known as gut flora. The number of individual

E. coli bacteria in the feces that a human excretes in one day averages between

500 billion and 10 trillion. All the different kinds of fecal coli bacteria, and all

the very similar bacteria that live in the ground are grouped together under the name

coliform bacteria. E. coli can be the causative agent of

several intestinal and extra-intestinal infections such as

urinary tract infections, meningitis, peritonitis, mastitis,

septicemia and gram-negative pneumonia.

Source: CDC: Center for Disease Control and Prevention

Tested by Kansas State University Inactivation Rate 99+%

Methicillin Resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) is a type of bacteria that is resistant

to certain antibiotics. These antibiotics include methicillin and other more

common antibiotics such as oxacillin, penicillin and amoxicillin. Staph infections, including

MRSA, occur most frequently among persons in hospitals and healthcare facilities

(such as nursing homes and dialysis centers) who have weakened immune

systems.

Source: CDC Centers for Disease Control and Prevention

Tested by Kansas State UniversityInactivation Rate 99+%

Bacillius Globigii

Bacillus globigii lives in soils around the world and can readily be found in samplings of

wind-borne dust particles. It is also known as Bacillus subtilis, its more modern name.

The National Institutes of Health's Centers for Disease Control lists BG as a "Class 1"

organism, meaning it is harmless and non-pathogenic to humans. It can be purchased

commercially and has been used for decades in biological studies. B. globigii has the

ability to form a tough, protective endospore, allowing the organism to tolerate extreme

environmental conditions, making it a perfect surrogate for

testing systems and procedures against Bacillus anthracis .

B. globigii is also often used as the Gram-positive equivalent

of Escherichia colii, an extensively studied Gram-negative

rod.

Information source: CDC (Center for Disease Control) and

Los Alamos National Laboratory

Tested by Kansas State University Inactivation Rate 99+%

Staphylococcus Aureus

Staphylococcus aureus, often referred to simply as "staph," is a bacteria commonly

found on the skin and in the nose of people. Person-to-person transmission is the usual

form of spread and occurs through contact with secretions from infected skin lesions,

nasal discharge or spread via the hands. S. aureus is a spherical bacterium (coccus)

which on microscopic examination appears in pairs, short chains, or bunched, grapelike

clusters. These organisms are Gram-positive. Some strains are capable of producing

a highly heat-stable protein toxin that causes illness in humans. Some isolates of

S. aureus are classified as Methicillin-resistant Staphylococcus aureus (MRSA). These

are a type of bacteria that are resistant to certain antibiotics. These antibiotics include

methicillin and other more common antibiotics such as

oxacillin, penicillin and amoxicillin. Staph infections, including

MRSA, occur most frequently among persons in hospitals

and healthcare facilities (such as nursing homes and dialysis

centers) who have weakened immune systems.

Information source: CDC (Center for Disease Control) and

FDA (Food and Drug Administration)

Streptococcus Pneumoniae

S. pneumoniae is an exclusively human pathogen and is spread from person-to-person

by respiratory droplets, meaning that transmission generally occurs during coughing or

sneezing to others within 6 feet of the carrier. Thus, carriers of S. pneumoniae, while

generally healthy, are an important source of infection and disease for others. The most

common types of infections caused by this bacteria include middle ear infections, pneumonia,

blood stream infections (bacteremia), sinus infections, and meningitis. In the

1940s, penicillin antibiotics became available and were used effectively to treat pneumococcal

infections. During the 1960s, however, the first

pneumococcal bacteria that were not susceptible ("resistant")

to penicillin were discovered in humans. Since then,

penicillin resistant pneumococcal bacteria have been reported

all over the world.

Tested by Kansas State University Inactivation Rate 99+%

Tested by Kansas State University Inactivation Rate 99+%

Pseudomonas Sp.

The bacterial genus Pseudomonas includes plant pathogenic bacteria such as P. syringae,

the opportunistic human pathogen P. aeruginosa, the ubiquitous soil bacterium

P. putida, and some species that are known to cause spoilage of unpasteurised milk and

other dairy products. The Pseudomonads are metabolically diverse, can consequently

colonize a wide range of niches, and are generally perceived to be agents of spoilage

and degradation.

Source: CDC: Center for Disease Control and Prevention

Tested by Kansas State University Inactivation Rate 99+%

3

4

Bacillus spp.

Bacillus cereus is a Gram-positive, facultatively aerobic sporeformer whose cells

are large rods and whose spores do not swell the sporangium. These and other

characteristics, including biochemical features, are used to differentiate and confirm

the presence of B. cereus, although these characteristics are shared with B. cereus

var. mycoides, B. thuringiensis and B. anthracis. B. cereus food poisoning is the

general description, although two recognized types of

illness are caused by two distinct metabolites. All people

are believed to be susceptible to B. cereus food poisoning.

Source: U.S. Food and Drug Administration

Tested by Kansas State University Inactivation Rate 99+%

Stachybotrys chartarum

Stachybotrys is a greenish-black fungus found worldwide that colonizes particularly

well in high-cellular material, such as straw, hay, paper, dust, lint, and cellulosecontaining

building materials such as fiber board and gypsum board that become

chronically moist or water damage due to excessive humidity, water leaks, condensation

or flooding. Stachybotrys chartaurum grows and produces spores in the

temperature range of 36-104F. It is also capable of producing

several toxins however, researchers still know

little about the temperature and moisture conditions

under which these toxins are produced.

Source: Health and Industry

Candida albicans

Candida albicans is a diploid sexual fungus (a form of yeast), and a causal agent

of opportunistic oral and vaginal infections in humans. Systemic fungal infections

have emerged as important causes of morbidity and mortality in immunocompromised

patients (e.g., AIDS, cancer chemotherapy, organ or bone marrow transplantation).

In addition, hospital-related infections in patients not previously considered

at risk (e.g. patients on an intensive care unit) have

become a cause of major health concern.

Source: CDC: Center for Disease Control and Prevention

Tested by Kansas State University Inactivation Rate 99+%

Tested by Kansas State University Inactivation Rate 99+%

Odors

The purpose of this test was to evaluate to what effect the RGF’s AOT unit has on

cleaning chemicals, pet odors and perfume odors. This test was performed utilizing

two 500 cubic foot test chambers and a ten-person odor panel. The qualitative assessments

of the ten-person odor panel were then used as a means to determine

the odor reduction.

Tested by C&W Engineering (Independent PE Firm)

Reduction %

Cleaning chemicals 55+%

Pet odors 72%

Perfume odors 63+%

5

Formaldehyde

The purpose of this test was to evaluate the effect the RGF AOT unit has on formaldehyde.

Tests were conducted in test chamber by Kansas State University

Formaldehyde

The purpose of this test was to evaluate the effect the RGF AOT unit has on formaldehyde

in a home.

Tests were conducted in actual mobile homes for FEMA.

Subway Corp. Ice Machine Test

The purpose of this test was to evaluate the effect the RGF AOT unit has on ice

machines used in Subway Sandwich stores

Tests were conducted in actual store.

Clean Ice Machine start of testing at

Subway Corp.

3 months later using RGF Ice Units no

visible microbial growth.

Before testing and cleaning visible

microbial growth.

6

Chemical odors (VOCs)

The purpose of this test was to evaluate the effect the RGF’s AOT unit has on chemical

odors.

Tests were conducted by GC/MS

Tested by NELAP Accredited Independent Lab

Mold/Yeast

The purpose of this test was to evaluate the effect the RGF AOT unit has on

mold/yeast bacteria (TPC). This test was performed utilizing a standard 2000 sq.

ft. home and 3000 sq. ft. simulated home.

Tested by California Microbiology Center

Independent Accredited Lab - IBR

Kansas State University

University of Florida

United Stares Air Force

R&D Labs

C&W Engineering

University of Cincinnati

Kane Regional Hospital

Reduction %

Toluene 29%

D-limonene 98%

Methyl Ethyl Ketone 13%

Reduction %

Bacteria 99%

Mold 97- 98%

Yeast 90+%

Chemical Compounds

Gas Chromatograph/Mass Spectrometer test performed by Nelap Accredited Lab

on airborne chemical compound reduction using RGF’s AOT.

Hydrogen Sulfide - Rotten eggs

Methyl mercaptan - Rotten cabbage

Carbon Disulfide - Vegetable sulfide

Butyl Acetate - Sweet banana

Methyl Metharcyline - Plastic

Tested by GC/MS Nelap Accredited Independent Lab

Reduction %

Hydrogen Sulfide 80%

Methyl mercaptan 100%

Carbon Disulfide 30%

Butyl Acetate 100%

Methyl Metharcyline 100%

7

Smoke (Odors and Particulates)

The purpose of this test was to evaluate to what effect the RGF’s AOT unit has on

cigarette smoke odors and particulate. This test was performed utilizing two 500

cubic foot test chambers and a ten-person odor panel. The qualitative assessments

of the ten-person odor panel were then used as a means to determine the odor reduction.

Particulate was tested with a laser particle counter.

Tested by C&W Engineering (Independent PE Firm)

Reduction %

Smoke odors 70%

Smoke particulate 25%

Particulate (REME only)

The REME Cell is also effective in reducing particle counts in the controlled chamber.

Particle counts are reduced to ISO Class 4 levels (10,000 - 0.1um) after 12 hours of

exposure to the REME Cell. After 24 hours of treatment, ISO Class 3 levels (1,000 -

0.1um) were achieved. These are better than HEPA results.

Tested By Kansas State University

Performance Analytical Labs

Reduction

12 hours ISO Class 4 (10,000 - 0.1um)

24 hours ISO Class 3 (1,000 - 0.1um)

Food Safety

RGF’s AOP Devices were approved by the USDA and FDA in 2001 for use in food

proccessing facilities worldwide. Since the approval RGF has had AOP equipment

in every aspect of food proccessing; meat, poultry, fish, grain, fruit, vegetables,

proccessed meats, ready to eat and restaurants.

Tested and approved for use in plants by USDA, FDA and FSIS.

Tested for safe reduction of airborne and surface bacteria, mold, virus and yeast in

food processing plants.

8

Electrical

All RGF AOP devices have been thourghly tested for electrical safety in house, by

consultants and certified independent agencies. Results were excellent.

Tested by: TUV, ETL, UL, NEI China, RGF Labs. The Japanese Government, GSA,

Electrical Power Research Institute.

Ozone / EMF

RGF AOP devices have been thourghly tested for ozone / emf - Electro Magnetic

Frequency and have passed Federal Safety Standards.

Tested by: FSIS Federal Safety Inspection Services

UL, ETL, TUV, CSA

ISSES / Disney

Note: Many household appliances emit some ozone and emf in safe low levels

such as and fluorescent lights, motors, computers, copy machines, refrigerators,

blenders,electronic air filters, air conditioners, electric fans, microwave ovens etc.

Effective Coverage (Area Test)

A 3000 sq.ft. simulated house was constructed inside a windowless

warehouse. The simulated house was constructd of virgin poly and was

completely emptied and then sanitized. A PHI cell was placed in the

center of the mock home to determine the effective area of coverage for

a single cell. Results were obtained showing

AOP levels of .01-.02 ppm in each room (results

will vary with virus, bacteria, organic,

VOC, and odor loading). It is highly unlikely

a cell will ever be used in a completely empty,

sanitized, and organic (load) free room. This

test was to demonstrate theoretical coverage.

In practice, one cell will effectively cover

2,000 square feet of an average home.

Tested by: RGF Labs, verified by independant P.E.

9

Sneeze Test - RGF PHI

A testing protocol concept was used which included a "Sneeze Simulation

Machine" and "Sneeze" chamber. A sneeze can travel at up to

100 mph, so we had to consider lung capacity, sneeze pressure, and

liquid volume to properly simulate a human sneeze. This was accomplished

and the test proceeded with outstanding results. An average of

78% reduction of microbials was achieved with PHI in a double blind

test, at 3 feet from the sneeze source. This is clearly not a medically supervised

test or protocol. However, from a practical point, it was definitely

providing some kill at the source and will provide some level of

protection.

Sneeze Test - REME

A testing protocol concept was used which included a "Sneeze Simulation

Machine" and "Sneeze" chamber. A sneeze can travel at up to

100 mph, so we had to consider lung capacity, sneeze pressure, and

liquid volume to properly simulate a human sneeze. This was accomplished

and the test proceeded with outstanding results. An average of

99% reduction of microbials was achieved with REME in a double blind

test, at 3 feet from the sneeze source. This is clearly not a medically supervised

test or protocol. However, from a practical point, it was definitely

providing some kill at the source and will provide some level of

protection.

3’

78% Reduction PHI

99% Reduction REME

Simulated Sneeze Lab Test at three feet in a 250 cu ft Bio Test

Chamber. An independent PE double blind study.

Tested by: Kansas State University, inactivation 99%

SAFETY

It is a normal reaction to question the long term safety of any product that is effective and uses new or "breakthrough" technology.

This type of question has become common as our litigious society has taught us to question things that significantly

outperform existing methods or products.

The RGF advanced oxidation technologies that produced the results found on the pages of this report certainly fall

into the category of breakthrough technology. This is evident by its outstanding test results across the entire range of microbes.

The breakthrough in the RGF advanced oxidation technologies is not found in the final product (hydroperoxides) but

rather in the method by which they are produced. The active ingredient created by the RGF products is a group of oxidants

known as Hydroperoxides. Hydroperoxides have been a common part of our environment for over 3.5 billion years. Hydroperoxides

are created in our atmosphere whenever three components are present: unstable oxygen molecules, water

vapor and energy (electro magnetic).

Hydroperoxides are very effective (as demonstrated by the test results in this book) at destroying harmful microbials.

As oxidants, they do this by either destroying the microbe through a process known as cell lysing or by changing its molecular

structure and rendering it harmless (which is the case in VOC's and odors). The amount of hydroperoxides required to accomplish

this task in a conditioned space is well below the level that is constantly in our outside air. The advanced oxidation

technology found in RGF's Guardian Air product family has brought the oxidants found in the outside air into the conditioned

space.

There is no known case of hydroperoxides ever creating a health risk. Considering we have been exposed to hydroperoxides

in nature since the day man stepped on the planet, it is a reasonable assumption that hydroperoxides do not

constitute a health risk. Over the past 20 plus years RGF has more than 1 million Advanced Oxidation products successfully

used worldwide.

Summary of university & independent lab tests

• 4-log reduction (99.99%) surface bacteria / virus reduction

• Over 80% VOC reduction

• 99% of microbes in human sneeze killed at 3 feet

• 97% airborne bacterial reduction

• 99% reductions of Ecoli, Listeria, Strep and MRSA

• 85% odor reduction

• 97% airborne mold reduction

• US Military approved for mold protection in field hospitals

• Hospital approvals Infectious Diseases - U.S. and International 99% reduction of Staph

(MRSA)

• 99% food surface microbial reduction

• Major US city school reports 20% reduction in absenteeism

• Tested and approved by the Chinese Government for protection against the SARS Virus

• Approved by the USDA, FSIS and FDA for use in food processing plants

• Fox News three-part indoor air series featured RGF and concluded substantial mold and

bacteria reductions

• RGF's technology has been featured on Fox, ABC, CBS and in Popular Science Magazine

RGF Guardian Air PHI Cell

An RGF Advanced Oxidation Technology

Effective on gases, odors and microbials

RGF Guardian Air REME Cell

An RGF Advanced Oxidation Technology

Effective on gases, odors, microbials

and particulate

10

Disclaimer:

All the above tests were performed on RGF Advanced Oxidation products with Advanced Oxidation Plasma of less than .02

ppm. They were conducted by independent accredited labs and university studies. They were funded and conducted by RGF’s

major clients to assure third party credibility. RGF products are not medical devices and no medical claims are made.

 

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